Differentiating Secondary Dengue Using IgG/IgM Antibody Ratio: A Cross-sectional Study

Introduction: Dengue is a viral infection with diverse clinical manifestations. Infection in most of the patients subclinical. It may also present as undifferentiated fever, severe dengue like Haemorrhagic Fever (DHF) and Shock Syndrome (DSS). Secondary predisposes to dengue, serious threat community they increase morbidities mortality. The diagnosis secondary either by Polymerase Chain Reaction (PCR) detect serotype or IgG/IgM antibody ratio. A ratio more than 1.1 evidence dengue. Aim: To find out prevalence measuring anti-IgG/IgM Enzyme Linked Immunosorbent Assay (ELISA) test. Materials Methods: This cross-sectional observational study was conducted at Government Kilpauk Medical College, Chennai for period six months from July 2021 December 2021. Sample size 450 calculated 95% confidence level, (CI 28.3-38.3) 33.3% prevalence. Blood samples were collected Nonstructural Protein 1 (NS1) antigen assay done. IgG IgM antibodies assays done NS1 positive individuals. who both IgM. Statistical analysis Package Social Sciences (SPSS) version 20.0. Percentage, mean standard deviation calculated. Results: Total included, which 194 positive, 106 males 88 females age 25±14.85 years. 89.17%. Two-tailed Fisher’s-exact test showed significant proportion among An >1.1 found 62.04%. association between thrombocytopenia [p-value=0.00504, Odd’s (OD) =3.316]. Conclusion: on third day symptom onset platelet count assist early thereby helps clinicians initiate appropriate treatment right time, reduce mortality rate decrease economic burden country.